Rose-Hulman Undergraduate Research Publications


Gaoshan Li

Document Type


Publication Date


First Advisor

Heather Chenette


Membrane adsorbers are one alternative to traditional bead-based chromatography that receive attention in the bioseparation process because of their ability to process material with lower residence times. However some properties of membranes make membrane adsorbers unrealistic for some applications. For example, some membrane adsorbers exhibit lower binding capacities compared with the traditional bead-based chromatography counterparts. The dynamic binding capacity (DBC) in this study is defined as the amount of protein bound in the column when the outlet reaches a certain concentration, which is 10% [2], and the equilibrium binding capacity (EBC) is the binding capacity under the equilibrium conditions, when the rate of protein adsorption is equal and opposite to the rate of protein desorption. Determining the dynamic binding capacities (DBC) of innovative protein adsorbers is one of the most important performance characterization steps in selecting appropriate materials for the purification process of biological products, such as monoclonal antibodies.

The goal of this research is to refine our previous methods and extend our analysis to determine DBC values using the existing DBC theory.


RHURP 18-03